Wintersemester 2014-2015

Mastermodul I Epigenetik

Vorbesprechung Mastermodul I: 21.10.2014 14-16 Uhr, Geb.60, Hom

Praktikumstermine Block Epigenetik: 24.11.-4.12.2014
Vorlesung: täglich 9.15 Uhr - 10.45 Uhr, A 4.3, SR
Praktikum: täglich 11.00 Uhr - ca. 17 Uhr, A 1.5, UG
Begleitseminar: täglich 10.45 Uhr - 11.15 Uhr
Abschlussseminar: 05.12.2014, A4.3, SR
Klausur: 12.12.2014, 12:00, großer Hörsaal, Geb. 61, Hom
Nachklausur: 14.04.2015, 15:00, Hörsaal 02, Geb. B2 1, SB
Prüfung(en): 10191, 10192

Praktikumsskript

pdf Skript



Praktikumsfiles

Excel-Dateien für die 450K-Auswertung

zip 450K_TP53-Netzwerk


doc genomische und Bisulfit-Sequenzen Backup-Amplikons


tdf-Dateien zum Hochladen in den IGV-Browser

[tdf] Methylierung_hg19

[tdf] Expression_hg18


Backups

zip Referenzsequenzen_BiQ

zip Reads_BiQ


ImageJ Installer

[exe] Image J Version 143

[exe] Image J Version 142



Folien Vorbesprechungen

ppt Einfuehrung_241114



Gelbilder

zip Jpegs



Sequenzierungsergebnisse

zip Reads


zip Reads (5000 max)



Gesamtergebnisse

[xlsx] Methylierung

[xlsx] RT-PCR



Lecture

„ Epigenetics “, compulsory for students of the Master Module I - open for others to participate on request

9.15 Uhr - 10.45 Uhr, A 4.3, Seminar room ground floor,

24.11.- 4.12.2014

Topics:
Introduction and overview
Definition of epigenetics and summary of basic epigenetic concepts and mechanisms of inheritance.

pdf Overview


Chromatin modifications I
Chromatin dynamics in the nucleus, Discrimination of Hetero- and Euchromatin, Composition and function and dynamics of the nucleosome. Methods to study chromatin. Introduction into histone variants and histone modification types.

pdf Chromatin I


Chromatin modifcations II
Introduction into basic reaction mechanisms: readers, writers and erasers of histone modifications. Models of epigenetic inheritance of histone modifications. Description of histone modifying and demodifying enzymes (Histone methyltransferases HMTs, Histone demethylases, Histone Acetytransferases and Deacetylases).
Methods to identify and locate chromatin modifications (ChIP, IF, MassSpec)

pdf Chromatin II


DNA-methylation
Description of various types of DNA-modifications. Mechanisms of estabilishment, maintenance and (passive and active) erasure.
Distribution and role of Tet oxigenases for DNA-methylation function and stability.
Distribution of DNA-methylation in the human genome. The role of DNA-methylation in epigenetic control of transcription – interplay with chromatin modifcations. Methods to identify and locate DNA-methylation. Dynamics of DNA-methylation in development and disease.

pdf DNA-Methylation


Epigenetics and development
Mechanisms of regrograming during early embryonic development. Mechanisms of imprinting and X-chromsomes inactivation
ACHTUNG Die Bilder sind gegnüber der Vorlesung umgestellt und durch Text ergänzt!!

pdf Epigenetics and Development


Epigenomics
Priniciples and experimental methods for genome wide methylation analysis (WGBS, RRBS, Tab-Seq, Cap-Seq), Chromatin modification analysis (ChIP-Seq) and analysis of open chromatin, ATAC, DNAseI, NOMe-Seq. Introduction into chromatin segmentation and ENCODE classifications. Future perspectives of chromatin 3D analysis (3C, 4C and Hi-C). Epigenomics data and functional genome interpretation. Description of the goals of the international human epigenome consortium IHEC.

pdf Epigenomics


Epigenetics and cancer
Contribution of genetic and epigenetic alterations to cellular dysfunction in cancer, basic concepts, examples of deregulation through changes in DNA-methylation. What defines a good molecular marker? From marker development to application; Epigenetic drugs and therapies.

pdf Epigenenetics & Cancer



pdf Summary



nach der Vorlesung Folien hier downloadbar