Wintersemester 2015-2016

Mastermodul I Epigenetik

Vorbesprechung Mastermodul I: 20.10.2015 14-16 Uhr, Geb.60, Hom

Praktikumstermine Block Epigenetik: 23.11.-3.12.2015

Vorlesung: täglich 9.15 Uhr - 10.45 Uhr, A 4.3, SR

Empfohlene Literatur zur Vorlesung:
Übersicht zu Epigenetik geben:
"Epigenetik" (Teil 4) in "Molekulare Genetik", Thieme Verlag 2015 (Hrsg.: Nordheim & Knippers) ISBN: 9783134770100
oder (ausführlicher)
"Epigenetics (Hrsg, Allis, Caparros, Jenuwein et al.), CSHL, 2015, 984S. ISBN: 978-1-936113-59-0

und speziell zu Krebs und Epigenetik

"Cancer Genetics and Epigenetics: Two Sides of the Same Coin?" Übersichtsartikel von You & Jones http://dx.doi.org/10.1016/j.ccr.2012.06.008

Praktikum: täglich 11.00 Uhr - ca. 17 Uhr, A 1.5, UG
Begleitseminar: täglich 10.45 Uhr - 11.15 Uhr
Abschlussseminar: 05.12.2015, A4.3, SR
Klausur: 11.12.2015, 13:00, Hörsaal, Geb. A1 7
Nachklausur:
Prüfung(en): 10191, 10192

Praktikumsskript

pdf Skript_ADV-I_Epigenetik



Praktikumsfiles

Excel-Dateien für die 450K-Auswertung

zip 450K_loci


Dateien zum Hochladen in den IGV-Browser

[bed] 450K_HCT116_WT_Replikat1

[bed] 450K_HCT116_WT_Replikat2

[bed] 450K_HCT116_DAC_Replikat1

[bed] 450K_HCT116_KO_Replikat1

[bed] 450K_HCT116_KO_Replikat2

[bed] 450K_HCT116_KO_DAC_Replikat1

[tdf] HT12v4_hg19


Primersequenzen

[xlsx] Selbstentworfene Primer


Backups

zip Referenzsequenzen_Backups


ImageJ Installer

[exe] Image J Version 143

[exe] Image J Version 142




Gelbilder

zip Gelelektrophoresen


Sequenzierungsergebnisse

zip Reads



Gesamtergebnisse

[xlsx] Ergebnistabelle


Lecture

„ Epigenetics “, compulsory for students of the Master Module I - open for others to participate on request

9.15 Uhr - 10.45 Uhr, A 4.3, Seminar room ground floor,

23.11.- 3.12.2015

Topics:
Introduction and overview
Definition of epigenetics and summary of basic epigenetic concepts and mechanisms of inheritance.

pdf Einfuehrung

pdf Epigenetics Overview


Chromatin modifications I
Chromatin dynamics in the nucleus, Discrimination of Hetero- and Euchromatin, Composition and function and dynamics of the nucleosome. Methods to study chromatin. Introduction into histone variants and histone modification types.
pdf Chromatin modifcations I



Chromatin modifcations II
Introduction into basic reaction mechanisms: readers, writers and erasers of histone modifications. Models of epigenetic inheritance of histone modifications. Description of histone modifying and demodifying enzymes (Histone methyltransferases HMTs, Histone demethylases, Histone Acetytransferases and Deacetylases).
Methods to identify and locate chromatin modifications (ChIP, IF, MassSpec)
pdf Chromatin modifcations II


DNA-methylation
Description of various types of DNA-modifications. Mechanisms of estabilishment, maintenance and (passive and active) erasure.
Distribution and role of Tet oxigenases for DNA-methylation function and stability.
Distribution of DNA-methylation in the human genome. The role of DNA-methylation in epigenetic control of transcription – interplay with chromatin modifcations. Methods to identify and locate DNA-methylation. Dynamics of DNA-methylation in development and disease.
pdf DNA-methylation


Epigenetics and development
Mechanisms of epigenetic regrograming in stem clls and during early embryonic development. Mechanisms of imprinting and germ cell development.
pdf Epigenetics & Development


Epigenomics
Priniciples and experimental methods for genome wide methylation analysis (WGBS, RRBS, Tab-Seq, Cap-Seq), Chromatin modification analysis (ChIP-Seq) and analysis of open chromatin, ATAC, DNAseI, NOMe-Seq. Introduction into chromatin segmentation and ENCODE classifications. Future perspectives of chromatin 3D analysis (3C, 4C and Hi-C). Epigenomics data and functional genome interpretation. Description of the goals of the international human epigenome consortium IHEC.
pdf Epigenomics


Epigenetics and cancer
Contribution of genetic and epigenetic alterations to cellular dysfunction in cancer, basic concepts, examples of deregulation through changes in DNA-methylation. What defines a good molecular marker? From marker development to application; Epigenetic drugs and therapies.
pdf Epigenetics and Cancer



nach der Vorlesung Folien hier downloadbar