Wintersemester 2015-2016

Principles of Epigenetics and Epigenomics

The exam date and location have been specified wrongly in the English version. See the update.

Results 1st Examen 24th February 2016
pdf Examensresults

Results Re-Examen 13th March 2016
pdf Re-Examensresults

The lectures provide an overview of epigenetic concepts and their relevance for human biology. Following an introduction into basic mechanisms and enzymologies of epigenetic control we will discus the developmental aspects of epigenetic modifications, particularly their importance for cell fate maintenance and cell function. Following a summary of disease related aspects we will briefly discus modern epigenomic mapping technologies, the basic principles of data managment and data interpretation.

Audience: Master Students (e.g. bioinformatics, biotechnology, biophysics and pharmacy) with basic knowledge in molecular genetics

Suggested Textbooks:
in English: "Epigenetics", by David Allis, Marie-Laure Caparros, Thomas Jenuwein, Danny Reinberg, Monika Lachlan, 2015, 984 pages, ISBN 978-1-936113-59-0
in German: "Molekulare Genetik" (mit einem Kapitel über Epigenetik), Hrsg.: Alfred Nordheim, Rolf Knippers, 10. Auflage 2015, 568 S. , 620 Abb. , ISBN: 9783134770100

Jörn Walter, Pavlo Lutsik, Karl Nordström, Martin Simon

Venue: Seminar Room 0.33, Building A2.4 (Genetics)

Time: WEDNESDAY 1:30pm (with one exception, see below)

Examen:24.02.2016 1:30pm (Seminar Room 0.33, Building A.2 4)
Re-Exam: 13.04.2016 1:30pm (Seminar Room 0.33, Building A.2 4)

L1: Jörn Walter 21.10.2015
Introduction and overview
Definition of epigenetic principles. Introduction into basic epigenetic concepts and mechanisms of inheritance.I mpact of epigenetic concepts for development. Epigenetics and cell biology. Introduction in epigenomics and relevance for biomedicine.
pdf Lecture 1 & Introduction

L2: Jörn Walter 28.10.2015
Chromatin mechanisms I
Basic principles of epigenetic modifications. Chromatin organisation: Organisation in the nucleus and in chromosomes, Discrimination of Hetero- and Euchromatin. Composition, function and dynamics of the nucleosome. Introduction into histone variants. Methods to study chromatin conformations.
pdf Lecture 2

L3: Jörn Walter 04.11.2015 NEW Time 6:00pm
Chromatin Mechanisms II
Description of histone modifications. Introduction in (main)histone modifying and demodifying enzymes: Histone methyltransferases (HMTs), Histone demethylases (HDMs), Histone Acetytransferases (HATs) and Deacetylases (HDACs). Introduction into basic reaction mechanisms: readers, writers and erasers of histone modifications. Models of epigenetic inheritance of histone modifications. Methods to identify and locate chromatin modifications (ChIP, IF, MassSpec). Histone modifications and regulation: Distribution and local control by chromatin modifications. Associations with Transcriptional control, DNA-Replication, DNA-Repair and DNA-Recombination.
pdf Lecture 3

L4: Jörn Walter 25.11.2015
Molecular control of DNA-methylation
Description of various types of DNA-modifications in bacteria, plants, animals and the human. Control of DNA-methylation by DNA-methyltransferases: Mechanisms of estabilishment, maintenance and (passive and active) erasure.
Distribution and role of Tet oxigenases for DNA-methylation function and stability.
Distribution of CpG methylation in the human genome. The role of DNA-methylation in epigenetic control of transcription – interplay with chromatin modifcations. Methods to identify and locate DNA-methylation. Dynamics of DNA-methylation in development and disease.
pdf Lecture 4 DNA-Methylation

L5: Jörn Walter 02.12.2015
Epigenetic reprogramming
Definition of major phases of epigenetic reprogramming during mammalian development focussing on demethylation in the zygote and germ cells.
Discussion of active and passive mechanisms and biological consequences.
pdf Lecture 5 Epigenetic reprogramming

L6: Jörn Walter 09.12.2015
Mechanisms of dosage compensation
Epigenetic control of dosage compensation. X-chromosome inactivation and genomic imprinting. Discovery and basic mechanisms of genomic imprinting. Genetic and epigenetic changes leading to human imprinting syndromes. Mechanisms of X-inactivation: Counting, choice, initiation and maintenance.
pdf Lecture 6 Mechanisms of dosage compensation

L7: Martin Simon 6.01.2016
pdf Lecture 7 RNA Interference

L8: Jörn Walter 13.01.2016
pdf Lecture 8 Epigenomics

L9: Karl Nordström/Abdhul Salhab 20.01.2016
pdf Lecture 9 Bioinformatic analysis of epigenomic data

L10: Jörn Walter 27.01.2016
pdf Lecture 10 Summary